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. 2017 Mar 3;7:44. doi: 10.1038/s41598-017-00121-y

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© The Author(s) 2017

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The fibronectin-binding ability of rNOX. The rNOX, rPGK, and E. coli total protein were applied to examine the fibronectin activity with indirect ELISA. Different amounts of proteins were coated in the 96 wells of ELISA plate. After washing and blocked with 5% BSA, 200 ng fibronectin in 100 μl PBST was added into each well and incubated for 1 h. And then the anti-fibronectin mAb and goat anti-mouse antibodies were sequentially overlaid to detect the binding activity. ***Represents very significant difference (p < 0.001), while “ns” represents no difference.