Table 1.
Oligonucleotide primers used for amplification and site-directed mutagenesis of NOX gene.
| Primers | Sequence (5′ → 3′) | Sites in Nox gene (nt) | Notes for the underlined |
|---|---|---|---|
| Pnox 1 | CCGGAATTCATGAAGATTATTTTAGTGGGAGCAAA | 1–26 | EcoR I site |
| Pnox 2 | ATTCACCACCTACCCAAACTGC | 133–154 | UGA to UGG |
| Pnox 3 | AGCAGTTTGGGTAGGTGGTGAAT | 132–154 | |
| Pnox 4 | GTTCGATTGGCCATGTACCGC | 335–355 | |
| Pnox 5 | GGCGGTACATGGCCAATCGAA | 334–354 | |
| Pnox 6 | TTTTACCTTTTTGCCAGAATGCTTCAG | 497–523 | |
| Pnox 7 | CTGAAGCATTCTGGCAAAAAGGTAAAA | 497–523 | |
| Pnox 8 | GCGTTTCCCCATGAACCAAT | 1180–1199 | |
| Pnox 9 | ATTGGTTCATGGGGAAACGCTATTCA | 1180–1205 | |
| Pnox 10 | GACAAGCTTTTAATATTTGTAGTCAATTCCTAATGCC | 1338–1365 | HindIII site |