Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Oligonucleotide primers used for amplification and site-directed mutagenesis of PGK gene.
| Primers | Sequence (5′ → 3′) | Sites in Nox gene (nt) | Notes for the underlined |
|---|---|---|---|
| P669-1 | CAGGTACCATGAAAAAAAGTATTGATGAT | 1–21 | Kpn I site |
| P669-2 | CAAATATATCACCCAATGATGCCCAATA | 409–436 | UGA to UGG |
| P669-3 | ATTGGGTGATATATTTGTTAATGATGCTTTTGGAA | 420–454 | |
| P669-4 | TTGGTCCATTCCACACAACTG | 935–955 | |
| P669-5 | AAACAGTTGTGTGGAATGGACCAATG | 932–957 | |
| P669-6 | ACGGATCCTTACTTATTTTCAATTGCACTA | 1164–1185 | BamH I site |
