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. 2017 Mar 2;7:113. doi: 10.1038/s41598-017-00155-2

Figure 4.

Figure 4

Introduction of 2 cysteines in the S4-S5L and S6T regions of hERG (D540C-L666C hERG) locks the channel closed in oxidative conditions. (A) Representative, superimposed recordings of the WT hERG current (3.6 µg plasmid plus 0.4 µg GFP plasmids) after 2 h incubation in Tyrode without (control) or with 0.2 mM tbHO2 (tbHO2). (B) D540C-L666C hERG channels, same as in A (3.6 µg plasmid plus 0.4 µg GFP plasmids). Cartoons: introduction of a cysteine is symbolized by a star. (C) Mean WT hERG tail-current density at −40 mV after a prepulse at +100 mV. (D) Activation curve obtained from the tail currents, using the protocol shown in (B). (E) Mean D540C-L666C hERG tail-current density at −40 mV after a prepulse at +100 mV, after 2 h incubation in Tyrode without (control) or with 0.2 mM tbHO2 (tbHO2), or after 2 h incubation in 10 mM DTT following tbHO2 incubation (2 h DTT). ***p < 0.001 versus control and ###p < 0.001 versus tbHO2, Mann-Whitney test.