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. 2017 Mar 2;7:113. doi: 10.1038/s41598-017-00155-2

Figure 5.

Figure 5

A cysteine disulfide bond reinforces the effect of the S4-S5L (+3) peptide, leading to full inhibition of the channel. (A) left, representative, superimposed recordings of the E544C-L666C hERG current (3.6 µg E544C-L666C hERG plasmid plus 0.4 µg GFP plasmids) and right, mean tail-current density at −40 mV after a prepulse at +100 mV after 2 h incubation in Tyrode without (control) or with 0.2 mM tbHO2 (tbHO2), both using the voltage protocol shown in inset. (B) left, representative, superimposed recordings of the single mutant L666C hERG current in the presence of E544C S4-S5L (+3) peptide (1 µg L666C hERG plus 3 µg peptide plasmids) and right, mean tail-current density  measured in the same conditions as A. (C,D) Mean tail-current density in the presence of only one cysteine, i.e. L666C hERG +S4-S5L (+3) peptide (C) or WT hERG +E544C S4-S5L (+3) peptide (D). ***p < 0.001 versus control, Mann-Whitney test.