Figure 6.

hERG inhibition provoked by E544C S4-S5_L (+3) peptide covalent binding to L666C mutant channel is reversible. Mean hERG tail-current density at −40 mV after a prepulse at +100 mV of L666C hERG channels in the presence of E544C S4-S5_L (+3) peptide (1 µg L666C hERG plus 3 µg peptide plasmids) after 2 h incubation in Tyrode without (control) or with 0.2 mM tbHO₂ (control and tbHO₂, respectively, same results as in Fig. 5B), and after 2 h or 10 min incubation in 10 mM DTT following tbHO₂ incubation, or after direct application of 10 mM DTT in the pipette tip, following tbHO₂ incubation. ***p < 0.001 versus control, ^##p < 0.01 and ^###p < 0.001 versus tbHO₂, Mann-Whitney test.