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. 2017 Mar 2;7:113. doi: 10.1038/s41598-017-00155-2

Figure 8.

Figure 8

A cysteine disulfide bond reinforces the effect of the activating S6T peptide, rendering the channel almost voltage independent. (A,B) Representative superimposed recordings of the single mutant D540C hERG current (A, 1 µg D540C hERG plus 3 µg GFP plasmids) and D540C hERG current in the presence of L666C S6T (−3) peptide (B, 1 µg D540C hERG plus 3 µg peptide plasmids) after 2 h incubation in Tyrode without (control) and with 0.2 mM tbHO2 (tbHO2), using the voltage protocol shown in inset. (C,D) Activation curve, with a double Boltzmann, obtained from the tail currents in the same conditions as A, in the absence (C) or presence of the L666C S6T (−3) peptide (D). (E) Maximum current density measured during the prepulse, using the protocol and conditions as in (A). (F,G) D540C hERG inactivation curve obtained using the protocol shown in right inset (same as in Fig. 2), in the absence (F) or presence of the L666C S6T (−3) peptide (G). *p < 0.05, **p < 0.01, ***p < 0.001, two way ANOVA with Bonferroni test.