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. 2017 Mar 3;7:51. doi: 10.1038/s41598-017-00142-7

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Characterisation of control and MERTK-RPE. (A1,2) Light micrograph of control and MERTK-RPE cells in culture (scale bar 50 μm). (B1,2) Electron micrograph of control and MERTK-RPE (scale bar 1 μm). (C1,2) Immunocytochemistry of iPSC derived RPE (scale bar 40 μm). (D) Immunohistochemistry of the control RPE and MERTK-RPE (scale bar = 10 μm). (E) RT PCR gel electrophoresis and Western blot of the MERTK gene and protein in the control RPE and MERTK-RPE. (F) gDNA sequence from MERTK fibroblasts of the exon 1/intron 1 junction of the MERTK gene compared to the corresponding MERTK-RPE derived cDNA sequence at the exon 1/exon 2 border. (G) gDNA sequence of MERTK fibroblasts compared to MERTK-RPE cDNA sequence in exon 14 of the MERTK gene.