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. 2017 Mar 7;7:92. doi: 10.1038/s41598-017-00145-4

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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CCL5 suppressed LPS-induced IL-6 and TNF-α production by peripheral blood monocytes from HD and CD patients in remission. (a,b) Peripheral blood monocytes from HD (n = 6) and CD patients (n = 7) were cultured in medium alone for 24 h; then, cells were washed and stimulated with or without CCL5 (pre-CCL5) for 24 h. Subsequently, cells were washed and stimulated with or without LPS. The levels of IL-6 and TNF-α in the supernatants were quantified by ELISA. Mann-Whitney U test was used for the comparisons between HD and CD patients (*p < 0.05, **p < 0.01).