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. 2017 Mar 8;7:118. doi: 10.1038/s41598-017-00118-7

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© The Author(s) 2017

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ARF1 regulates crystal cell- mediated melanization and phenoloxidase activity. (A–F) Photomicrographs showing posterior region of third instar larvae of specific genotypes (A) e33cGal4 (B) UAS arf1rnai (C) UAS arf1rnai/e33cGal4 (D) UAS arf1 (E) UAS arf1/e33cGal4 (F)UAS arf1rnai/UASarf1rnai; UAS arf1/e33cGal4 that were heated at 60 °C for 10 min to visualize the melanization response. (G) Melanized crystal cells were quantified and represented graphically. (H) Graph representing phenoloxidase activity in the hemolymph of the indicated genotypes (detected as absorbance at 490 nm) after conversion of L-3, 4-dihydroxyphenylalanine. Scale Bar: (A–F) 100 μm. Number of larvae analyzed per genotype (n = 10). Error bars show standard error of mean. P-value: ** and *** indicate P < 0.01 and P < 0.001 respectively.