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. 2017 Mar 6;7:76. doi: 10.1038/s41598-017-00135-6

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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De novo induced prion particles are infectious. (a) Donor lysates from cultures induced for the indicated times (x-axis) were transfected into [psi ⁻][PIN ⁺] cultures. Transfection conversion frequencies are shown. (b) The same donor cultures, used in transfection, were also assessed for the ability to form Sup35PrD-GFP aggregates (rings, lines, and dots; grey bars) and de novo induction frequency (white bars). This data represents single trial out of three independent trials.