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. 2017 Mar 22;7:231. doi: 10.1038/s41598-017-00159-y

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Evaluation of ROS generation. Images (A–L) represent images of intracellular ROS generation, 4′,6-diamidino-2-phenylindole (DAPI) fluorescence and merged images of ROS generation and DAPI of cumulus oocyte complex exposed to D-galactose (Images D–F), galactitol (Images G–I), and Gal 1-P (galactose 1-phosphate) (Images J–L), and untreated control (Images A–C). Scale bars: 100 μm. Images shown are from a typical experiment performed at least three times.