Skip to main content
. 2017 Mar 23;7:325. doi: 10.1038/s41598-017-00282-w

Figure 3.

Figure 3

PRI-724 induces fibrolytic inflammatory cells expressing MMP-8. HCV transgenic mice were treated intraperitoneally with vehicle or PRI-724 once per day for 42 days at a dose of 5 or 20 mg/kg body weight or 1 week ON/OFF at a dose of 5 mg/kg body weight for 6 weeks (total of three cycles, each consisting of 1 week of once-daily dosing followed by 1 week without dosing) (Fig. 1A). Undosed non-trangenic mice were used as controls. (A) Expression of F4/80, Ly-6C, and Gr-1 was examined by immunohistochemistry with anti-F4/80, -Ly-6C, and -Gr-1 antibodies, respectively; representative micrographs are provided (scale bars = 200 μm). (B) Expression of MMP-8 (green) and cellular markers (red) F4/80 (left panels) and Gr-1 (right panels) was examined by immunofluorescent staining. Nuclei were counterstained with DAPI (blue). Representative images are provided (scale bars = 100 μm). MMP-8-producing cells in the PRI-724-treated group are boxed, and enlarged images are provided to the right of each original image (scale bars = 50 μm).