Fig. 1. Top: NCL synthesis of CaM thioproteins. Cys135 is masked as glutamine by alkylation with iodoacetamide. Middle: synthesis of GB1 thioproteins by hydrazide ligation. Cys24 undergoes desulfurization to the native residue Ala24. Bottom: total solid phase synthesis of collagen strands by POG trimer and thioamide precursor couplings. Upon completion of synthesis, triple helices are formed by incubation of the monomers at 4 °C.
