Table 2. GB1 thermodynamic values.

GB1 variant	T m (°C)	ΔT M (°C)	ΔΔG U a (kcal mol–1)
Oxo	78.2 ± 0.0	—	—
LeuS 5	65.6 ± 1.6	–12.6	–2.5 ± 0.3
IleS 6	68.7 ± 0.5	–9.5	–1.9 ± 0.1
LeuS 7	75.9 ± 1.2	–2.3	–0.5 ± 0.2

^a Change in unfolding free energy, ΔΔG _U, calculated from ΔT _M using eqn (S8) as described in the ESI.