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| Collagen variant | T M (°C) | ΔG U a | ΔΔG U a | t 1/2 (min) |
| Pro-Pro-Gly Oxo | 36.7 ± 0.4 | 10.8 | 32.5 ± 2.0 | |
| ProS-Pro-Gly | 11.0 ± 0.9 | 5.1 | –5.7 | 25.0 ± 4.5 |
| Pro-ProS-Gly | 38.5 ± 0.5 | 11.2 | 0.4 | 35.3 ± 5.2 |
| Pro-Pro-GlyS | 30.3 ± 0.5 | 9.2 | –1.6 | 40.3 ± 7.6 |
| Pro-Hyp-Gly Oxo | 39.9 ± 0.5 | 12.0 | 24.0 ± 4.5 | |
| Pro-HypS-Gly | 41.0 ± 0.2 | 12.6 | 0.6 | 17.7 ± 4.0 |
| Pro-Hyp-GlyS | 34.5 ± 0.0 | 11.1 | –0.9 | 23.8 ± 2.8 |
a Unfolding free energy in kcal mol–1, calculated as described in the ESI.
