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. 2017 Mar 10;7:139. doi: 10.1038/s41598-017-00213-9

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Effect of chronic E2 exposure on the gene expression of ET-1 and its receptors in the RVLM and PVN. The mRNA expression levels of ET-1, ET_A and ET_B receptor and Angiotensin II type 1 receptor in the RVLM and PVN of control and E2 treated rats are shown in (A–H). The fold change was calculated relative to β-actin by the comparative C_t method using 2^−ΔΔCt. The C_t values of all the groups were normalized to control rats (n = 4–5 per group). *Denotes significant difference (p < 0.05) from control group.