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. 2017 Mar 10;7:139. doi: 10.1038/s41598-017-00213-9

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Effect of chronic E2 exposure on protein levels of ET_A in the PVN. Sample blots and densitometry results from western blot analysis of ET_A in the PVN of control and E2-treated rats are shown. Bar graphs represent mean ± SE for 4–5 animals. *Indicates significant difference from control animals.