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. 2017 Mar 15;7:199. doi: 10.1038/s41598-017-00241-5

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Identification of human retinal progenitor cells (HRPCs). (a–o) Five markers including Ki67, Pax6, Sox2, Nestin and GFAP were used to identify the characteristics of HRPCs in passages1, 3 and 5. (p–s) Flow cytometry analysis of HRPCs with Pax6, Sox2, Nestin and GFAP. (t) Corresponding statistic analysis of (a–o). For Ki67 staining, the significance between P1 and P3 is P = 0.004637. The significance between P1 and P5 is P = 0.000349. The significance between P3 and P5 is P = 0.028506. For SOX2 staining, the significance between P1 and P3 is P = 0.010751. The significance between P1 and P5 is P = 0.000505. The significance between P3 and P5 is P = 0.020324. For GFAP staining, the significance between P1 and P5 is P = 0.000635. The significance between P3 and P5 is P = 0.005400. *P < 0.05; **P < 0.01; ***P < 0.001.