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. 2017 Mar 15;7:199. doi: 10.1038/s41598-017-00241-5

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© The Author(s) 2017

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Identification of human bone mesenchymal stem cells (HBMSCs). (a–d) Flow cytometry analysis of HBMSCs with CD44, CD73, CD90 and CD105. (e–h) Characteristics of HBMSCs. (e,f) Bone-forming test, results showed that there were Alizarin Red S staning mineralized matrixes formed. (g) Adipocyte-differentiating test showed that lipid vesicles were visualized with Oil Red O. (h) Cartilage-forming test showed that Alcian Blue staining cartilage could be visualized. (i) Flow cytometry analysis of negative marker cocktail. Results showed that CD11b, CD19, CD34, CD43 and HLA-DR were negative in the HBMSCs. (j,k) Identification of GFP-lentivirus transfected HBMSCs. (k) Image of GFP showed that more than 80% cells were GFP positive. (j) Flow cytometry analysis of HBMSCs showed that over 90% cells were positive for GFP.