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. 2017 Mar 15;7:199. doi: 10.1038/s41598-017-00241-5

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Comparison of out nucleus layer (ONL) thickness after single and combined cell transplantation into subretinal space (SRS). (a,f,k,p) Representative ONL thickness of untreated RCS rats on 3, 6, 9 and 12 weeks (the same time point with operational groups). (b,g,l,q) Representative image of ONL thickness after PBS injection on 3, 6, 9 and 12 post operational weeks. (c,h,m,r) Representative ONL thickness after human retinal progenitor cells (HRPCs) injection on 3, 6, 9 and 12 post operational weeks. (d,i,n,s) Representative ONL thickness after human bone mesenchymal stem cells (HBMSCs) injection on 3, 6, 9 and 12 post operational weeks. (e,j,o,t) Representative ONL thickness after HRPCs and HBMSCs double injection on 3, 6, 9 and 12 post operational weeks. (q) Corresponding statistical analysis of the ONL thickness in all 5 groups at 4 time points. Results showed that all cell transplantation groups displayed increases of the ONL thickness, which indicated the protection of photoreceptor. Combined transplantation showed longest photoreceptor-protection effect. *P < 0.05; **P < 0.01; ***P < 0.001. (n = 3).