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. 2017 Mar 14;7:178. doi: 10.1038/s41598-017-00264-y

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© The Author(s) 2017

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Back pressure and recovery of IgG on the ProA-Column and ProA-SpM. (a) Comparison of back pressure on LC eluted by 50 mM phosphate buffer with 150 mM NaCl as a mobile phase using the ProA-Column or ProA-SpM. (b) Comparison of the recovery for IgG with the ProA-Column or ProA-SpM, total amount was estimated by sum of the peak area among the flow though peak and IgG.