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. 2017 Mar 14;7:178. doi: 10.1038/s41598-017-00264-y

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Affinity separation of cell supernatant for isolation of IgG. (a) UV chromatograms of a supernatant in a protein A load sample and a standard IgG 1 with the ProA-SpM. (b) Total ion chromatograms in RPLC.