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CD46 depletion enhances auto-IgG-mediated C3 deposition in HaCaT cells. HaCaT cells transfected with CD46 siRNA were incubated with purified auto-IgG overnight at 37 °C. Fresh healthy serum containing complement components was added to mimic BP pathogenesis. C3 deposition was detected by IF staining to determine the complement activation level. Scale bar, 25 nm. Auto-IgG, pathogenic IgG from BP patients’ serum; NHS, normal human serum; NC, negative control siRNA.
