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. 2017 Mar 10;7:140. doi: 10.1038/s41598-017-00237-1

Figure 3.

Figure 3

The effect of Rho kinase inhibition on stress-fiber formation and FN fibrillogenesis. (a) Representative images of immunocytochemistry of hUASMCs exposed to PHP (110–180 kPa, 0.002 Hz) for 24 h. Cells were incubated with Y27630 (40 μmol/L) or C3 (10 μmol/L) during pressurization and stained for F-actin and FN. Scale bars: 50 μm. (b and c) Quantification of intensity of F-actin and FN of (a), respectively. n = 7–11, NS, not significant vs. 101 kPa, 0.0 Hz (atmospheric pressure); **p < 0.01; ***p < 0.001. Values are shown as the mean ± standard error of the mean (SEM) of independent experiments. Data were analyzed using analysis of variance (ANOVA) followed by Dunnett’s multiple comparison test.