Figure 5.

Identified targets are regulated in vivo by PPARα. (A) Read pileup profiles for CR and HFD PPARα ChIP-Seq near known regulated genes with corresponding in vivo qPCR results following fenofibrate treatment. (B) Read pileup profiles for CR and HFD PPARα ChIP-Seq near novel regulated genes with corresponding in vivo qPCR results following fenofibrate treatment. Read pileup refers to extended, normalized, and smoothed read pileup counts extracted from concatenated pools of aligned reads for the biological replicates for each factor (see Methods). Green lines indicate significantly called peaks in both CR and HFD. Red and blue lines indicate significantly called peaks in HFD and CR, respectively. *p < 0.05, **p  < 0.01.