Abstract
Patterns of accumulation and ontogenetic relationships among proteins of cotton (Gossypium hirsutum L.) seeds were examined between 10 days postanthesis and maturity (56 days). Total and extractable nitrogen contents were determined; alkali- and water-soluble proteins were assayed quantitatively and electrophoretically. Two alkali-soluble proteins present in the electrophoretogram of mature embryos first appeared at 21 days postanthesis; most of the final profile was established by 28 days. Except for minor changes centering around the 7th week of development, the pattern from 28 days to maturity was marked by intensification of bands. The quantity of water-soluble nitrogen increased through the first 21 days of development, then declined until 42 days, when it again began to increase; it reached its highest level at maturity. There was evidence of a high peptide content 7 weeks postanthesis.
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- Fairbanks G., Steck T. L., Wallach D. F. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry. 1971 Jun 22;10(13):2606–2617. doi: 10.1021/bi00789a030. [DOI] [PubMed] [Google Scholar]
- Hill J. E., Breidenbach R. W. Proteins of Soybean Seeds: II. Accumulation of the Major Protein Components during Seed Development and Maturation. Plant Physiol. 1974 May;53(5):747–751. doi: 10.1104/pp.53.5.747. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hill J. E., Breidenbach R. W. Proteins of soybean seeds: I. Isolation and characterization of the major components. Plant Physiol. 1974 May;53(5):742–746. doi: 10.1104/pp.53.5.742. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ihle J. N., Dure L. S., 3rd The developmental biochemistry of cottonseed embryogenesis and germination. 3. Regulation of the biosynthesis of enzymes utilized in germination. J Biol Chem. 1972 Aug 25;247(16):5048–5055. [PubMed] [Google Scholar]
- King E. E., Lamkin G. E. Isolation of proteins from glanded cottonseed. J Agric Food Chem. 1977 Sep-Oct;25(5):1211–1213. doi: 10.1021/jf60213a039. [DOI] [PubMed] [Google Scholar]