Table 2.

Numerical data as obtained from PCR amplification by RAPD primers among Costus pictus accessions

Sr. no.	Primer name	Primer sequence 5′–3′	GC content (%)	Annealing temperature	Total number of bands	Total number of loci	Monomorphic loci	Polymorphic loci	Polymorphism %	PIC	Molecular weight (bp)
1	OPK-07	AGCGAGCAAG	60	36	133	10	8	2	20.00	0.89	220–1500
2	OPK-16	GAGCGTCGAA	60	36	283	24	15	9	37.50	0.95	160–2670
3	OPK-18	CCTAGTCGAG	60	36	159	14	8	6	42.86	0.92	190–2580
4	OPK-20	GTGTCGCGAG	70	38	154	16	8	8	50.00	0.91	280–2200
5	OPM-01	GTTGGTGGCT	60	36	184	17	10	7	41.18	0.93	230–2640
6	OPM-02	ACAACGCCTC	60	36	128	12	7	5	41.67	0.90	220–1720
7	OPM-03	GGGGGATGAG	70	38	121	13	7	6	46.15	0.89	260–2930
8	OPM-04	GGCGGTTGTC	70	38	144	11	8	3	27.27	0.91	160–1800
9	OPM-05	GGGAACGTG	67	37	156	14	8	6	42.86	0.90	120–2200
10	OPM-06	CTGGGCAACT	60	36	139	12	8	4	33.33	0.90	130–3125
11	OPM-07	CCGTGACTCA	60	36	138	12	7	5	41.67	0.90	245–2065
12	OPM-08	TCTGTTCCCC	60	36	151	13	7	6	46.15	0.91	240–2100
13	OPM-09	GTCTTGCGGA	60	36	162	13	10	3	23.08	0.91	160–2900
14	OPM-14	TCTGGCGCAC	70	38	115	9	7	2	22.22	0.88	110–1350
15	OPN-02	ACCAGGGGCA	70	38	109	9	6	3	33.33	0.87	110–2200
16	OPN-04	GACCGACCCA	70	38	245	19	14	5	26.32	0.94	141–1600
17	OPN-06	GAGACGCACA	60	36	128	12	7	5	41.67	0.90	190–3110
18	OPN-07	CAGCCCAGAG	70	38	198	19	11	8	42.11	0.93	140–1050
19	OPN-08	ACCTCAGCTC	60	36	92	7	5	2	28.57	0.85	390–1410
20	OPN-09	TGCCGGCTTG	70	38	154	15	9	6	40.00	0.91	240–2480
21	OPN-10	ACAACTGGGG	60	36	239	20	14	6	30.00	0.94	140–1860
22	OPN-13	AGCGTCACTC	60	36	102	9	6	3	33.33	0.87	330–2160
23	OPN-15	CAGCGACTGT	60	36	166	14	10	4	28.57	0.92	200–2420
24	OPN-16	AAGCGACCTG	60	36	149	14	8	6	42.86	0.91	250–3190
25	OPN-18	GGTGAGGTCA	60	36	194	15	11	4	26.67	0.93	170–3000
Total					3943	343	219	124	–	–	–
Average/primer					157.72	13.72	8.76	4.96	35.57	0.91	110–3190a

^aRange