Table 5.

Numerical data as obtained from PCR amplification by ISSR primers among Costus pictus accessions

Sr. no	Primer name	Primer sequence 5′–3′	GC content  %	Annealing temperature	Total number of bands	Total number of loci	Monomorphic loci	Polymorphic loci	Polymorphism %	PIC value	Molecular weight (bp)
1	UBC 808	AGAGAGAGAGAGAGAGC	53	60	99	8	3	5	62.50	0.87	370–2025
2	UBC 809	AGAGAGAGAGAGAGAGG	53	60	104	11	6	5	45.45	0.88	380–2190
3	UBC 811	GAGAGAGAGAGAGAGAC	53	63	60	6	3	3	50.00	0.79	450–2515
4	UBC 812	GAGAGAGAGAGAGAGAA	47	51	63	5	4	1	20.00	0.77	250–1700
5	UBC 818	CACACACACACACACAG	53	53	253	13	6	7	53.85	0.91	260–2500
6	UBC 826	ACACACACACACACACC	53	53	102	11	8	3	27.27	0.90	200–2220
7	UBC 834	AGAGAGAGAGAGAGAGYT	44	52.5	113	10	6	4	40.00	0.88	320–1880
8	UBC 835	AGAGAGAGAGAGAGAGYC	50	52.5	91	7	5	2	28.57	0.85	400–1790
9	UBC 841	GAGAGAGAGAGAGAGAY	47	52.5	88	7	4	3	42.86	0.84	640–2830
10	UBC 842	GAGAGAGAGAGAGAGAYG	50	53	122	9	4	5	55.56	0.89	355–2400
11	UBC 847	CACACACACACACACARC	50	53	86	8	4	4	50.00	0.85	610–3070
12	UBC 848	CACACACACACACACARG	50	52.5	101	8	6	2	25.00	0.86	335–2190
13	UBC 852	GTGTGTGTGTGTGTGTYC	50	52	116	10	4	6	60.00	0.89	305–2725
14	UBC 866	CTCCTCCTCCTCCTCCTC	67	57.5	94	9	4	5	55.56	0.87	305–2830
15	UBC 880	GGAGAGGAGAGGAGA	60	55.5	115	9	6	3	33.33	0.88	400–2580
16	UBC 883	TATATATATATATABVB	0	36.8	105	8	5	3	37.50	0.87	400–2180
17	UBC 888	CACACACACACACABDB	41	50.5	105	8	6	2	25.00	0.87	390–2000
18	UBC 889	ACACACACACACACACDBD	42	51.5	108	11	4	7	63.64	0.89	385–2560
19	UBC 890	CTCTCTCTCTCTCTVHV	41	51	115	9	6	3	33.33	0.88	305–2770
20	UBC 891	TGTGTGTGTGTGTGHVH	41	51	121	10	6	4	40.00	0.89	310–2680
Total					2161	177	100	77	–	–	–
Average/primer					108.05	8.85	5	3.85	42.47	0.87	200–3070a

^aRange