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Expression of different members of the MKP family upon LH stimulation. Ovarian follicles were exposed to LH for 0, 1, 2, 3, and 4 h. At the end of incubation, RNA was extracted, and cDNA was synthesized and subjected to Q-PCR for MKP-1 (DUSP1), MKP-2 (DUSP4), MKP-3 (DUSP6), MKP-X (DUSP7), and PAC1 (DUSP2) transcripts. The graph shows means ± se of three independent experiments (*, P < 0.03; **, P < 0.005).
