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Agonist-selective patterns of sst2A phosphorylation. HEK293 cells stably expressing sst2A were transfected with empty vector (MOCK), GRK2, GRK3, or GRK5 for 2 d. Cells were then treated with either 1 μm SS-14 or 10 μm SOM230 for 5 min. The levels of phosphorylated sst2A receptors were determined using anti-psst2A(Thr356/Thr359) (upper panel) and anti-psst2A(Thr353/Thr354) (lower panel) antibodies. The positions of molecular mass markers are indicated on the left (in kilodaltons).
