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Sialic acid modification is not required for multimer formation or secretion of human or mouse adiponectin. A, WT and Lec2 CHO cells were transiently transfected with human (Hu) or mouse (Mo) adiponectin, and secreted adiponectin was analyzed by SDS-PAGE and Western blotting. B, Velocity sedimentation analysis of human adiponectin secreted from WT and Lec2 CHO. C, 2DE of WT and Lec2 samples. Data are representative of three independent experiments. H, Hexamers; T, trimers.
