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Effect of APN on LPS-mediated AF iNOS mRNA and protein expression and NO production in cultured medium. A, iNOS mRNA expression. B, iNOS protein expression. Expression of β-actin band was used as a loading control. C, NO production in cultured medium. **, P < 0.01 vs. con; ##, P < 0.01 vs. LPS. Values are means ± sem. con, Control group.
