Fig. 1.
GPR119 is responsive to lipid amides. A, Adenylyl cyclase assay in human GPR119-transfected HEK293 cells. HEK293 cells were transfected with 5 μg of plasmid DNA encoding haemmaglutinin (HA)-tagged human GPR119 in a 10-cm dish. Twenty-four hours after transfection, cells were collected, and 1 × 105 cells per well were used for cAMP assay. Data were shown as picomole cAMP production by cells plated in one well of a 96-well plate (mean ± sem, n = 3) and plotted using Prism software program. B, Comparison between GPR119 synthetic agonist AR231453, OLDA, C18:1 LPC, and platelet-activating factor (PAF) on human GPR119-mediated cAMP production in transfected 293 cells.