Fig. 6.

Expression of TH, DDC, and FAAH in mouse islets and intestines. RNA was extracted from whole brain, hypothalamus, indicated regions of the gut, and pancreas of C57bl/6 mice (n = 3) and from isolated pancreatic islets of C57bl/6, ob/ob, and db/db mice. Expression of TH and DDC was analyzed by Taqman QPCR (qRT-PCR). Expression levels were normalized with internal β-actin control and shown for TH (A) and for DDC (B). FAAH expression in the comparable sets of mouse tissues (C) was determined using a custom Affymetrix gene expression chip. QRT-PCR, Quantitative real-time RT-PCR; SK, skeletal; Sm, small.