Fig. 1.

Mapping TSSs for hGHR V2. A, Representative autoradiograph of size-fractionated products of primer extension reactions carried out with a primer (V2PE) complementary to the V2 exon sequence and 20 μg total RNA extracted from three human cell lines and liver tissues. The sizes of two specific products (arrows) were determined by a concurrently electrophoresed ³²P-labeled φX174 HinfI DNA ladder (lane M). Lane 1, HEK293 cells; lane 2, HEK293 cells transfected with V2(−2623/+331); lane 3, Huh7 cells; line 4, SGBS preadipocytes; lane 5, human fetal liver; lane 6, human adult liver. This assay was repeated three times with RNAs from at least two different cell pools and tissue samples. B, Positions corresponding to the two longest extended V2 5′-cDNA ends in our primer extension assays are shown in bold italic and underlined. The major (T, bold, boxed) and minor TSSs for ovine 1B are indicated by solid circles. The major (G, bold, underlined) and minor TSSs identified for bovine 1B are marked by open circles. The oligonucleotide primer (V2PE) used for primer extension is underlined by an arrow.