Fig. 6.

Schematic representation of the hGHR V2 exon and its 5′-upstream promoter. This diagram shows the hGHR V2 and its promoter region spanning from 720 bp upstream to 362 bp downstream of the designated TSS (+1), with the identified proximal promoter, core promoter, and activation as well as inhibitory domains. No TATA box was found within the V2 promoter region, whereas an INR-like element surrounds the TSS (+1). Putative cis-elements, including a CCAAT box, CHOP, and c- Ets1 binding sites, were found within a 40-bp region approximately 50 bp upstream of the TSS. Two putative Hes1 binding sites are present within the V2 exon as well as putative binding sites for Sp, SREBP, ZBP-89, and Egr-1 that are located as a cluster within an approximately 20-bp region.