Fig. 1.

Sequence comparison of the exon and 5′-flanking sequences of human V2, ovine 1B, bovine 1B, and mouse L2. The human V2 sequence (GenBank AF322014) containing 211 bp of the 5′-upstream promoter region and about 300 bp of V2 exon is aligned with its homologs, including ovine 1B (S78252), bovine 1B (AF046861), and mouse L2 (AF120480). Sequence identity is indicated by asterisks, whereas dashes indicate gaps introduced to maximize alignment. The major TSS of ovine 1B (T, bold) is indicated by +1 with an arrow. This is also used as position +1 for our human V2 promoter construct numeration because it represents the longest V2-like cDNA identified to date. Motifs of potential binding sites for transcription factors are underlined and named. The highly conserved CCAAT box and two overlapped Ets binding sites are boxed. The human-specific CHOP and Hes1 sites are marked with a dashed line and round dots, respectively. Sp sites identified in ovine and bovine 1B studies are indicated (bold and italicized). The SREBP (italicized) and ZBP-89 (arrow) sites overlap with the exon Sp site.