Fig. 4.

Interactions between CHOP and Ets1 in regulating V2 promoter activity. COS-1 cells were cotransfected with V2(−211/+362) or V2(−211/+71) and 0.05 μg Ets1 plus increasing amounts (0.05, 0.1, and 0.2 μg) of CHOP and assayed for V2 transcriptional activities. The total amount of plasmid was adjusted to 1 μg/transfection using an empty vector. Data are expressed as fold activation relative to no CHOP or Ets1 overexpression (0 ng CHOP plus 0 ng Ets), which is set as 1. Significant differences between paired values are shown: *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, P > 0.05. Data are presented as mean ± se from n = 3–5 different experiments.