Figure 4.

FSCN1 is a direct miR-539 target. (A) FSCN1 3′-UTRs contains one predicted miR-539 binding site. In the figure, the alignment of the seed region of miR-539 with the FSCN1 3′-UTR is shown. The sites mutated by target mutagenesis are indicated below. (B) Relative luciferase activity was detected by luciferase reporter assays. (C) Quantification of FSCN1 mRNA expression by qRT-PCR. D) FSCN1 protein expression was assessed by western blotting after miR-539 overexpression. **P<0.01. Data are presented as the mean ± SD; P-values was calculated with Student's t-test.