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. 2017 Apr 3;37(5):2593–2602. doi: 10.3892/or.2017.5549

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Copyright: © Liu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Restoring FSCN1 expression reverses the effect of miR-539 on HCC cell migration and invasion. SK-HEP1 cells were transfected with pcDNA3-FSCN1 alone, pcDNA3-FSCN1 plus LV-miR-539, and the corresponding pcDNA3 vector as the negative control. (A) Detection of FSCN1 protein expression by western blot analysis. (B and C) Effects of pcDNA-FSCN1 on miR-539-infected SK-HEP1 cells were examined by Transwell migration assays. Scale bar indicates 200 µm. The graph shows the mean ± SD, and **P<0.01, as determined by Student's t-test, for the test groups versus the vector control. (D and E) Transwell invasion assays of LV-miR-539-infected SK-HEP1 cells co-transfected with pcDNA-FSCN1. Scale bar indicates 200 µm. Data are presented as the mean ± SD; P-values was calculated with Student's t-test.