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. 2017 Mar 15;7:191. doi: 10.1038/s41598-017-00301-w

Figure 2.

Figure 2

MKL1 deficiency dampens Rel-A/p65 dependent transcription. (A,B) THP-1 cells were transfected with indicated siRNAs followed by treatment with TNF-α (10 ng/ml) for 6 hours. mRNA (A) and protein (B) levels of pro-inflammatory mediators were measured by qPCR and ELISA. (C,D) THP-1 cells were transfected with indicated siRNAs followed by treatment with LPS (100 ng/ml) for 6 hours. mRNA (C) and protein (D) levels of pro-inflammatory mediators were measured by qPCR and ELISA. (E) WT or MKL KO MEF cells were transfected with a generic κB reporter construct followed by treatment with TNF-α (10 ng/ml) for 6 hours. Data are expressed as RLU. (F–I) MEF cells were treated with TNF-α (10 ng/ml). mRNA (F) and protein (G) levels of pro-inflammatory mediators were measured by qPCR and ELISA. (H) Heat map and (I) Go analysis.