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. 2017 Mar 15;7:191. doi: 10.1038/s41598-017-00301-w

Figure 3.

Figure 3

A reciprocal interplay between MKL1 and p65 on the chromatin. (A,B) THP-1 cells (A) or differentiated primary human macrophages (B) were treated with TNF-α (10 ng/ml) for 6 hours. ChIP assays were performed with indicated antibodies. Precipitated DNA was amplified with indicated primers shown with conserved binding motifs. κBRE, NF-κB response element; SRE, serum response element; TSS, transcription start site (C,D) THP-1 cells (C) and differentiated primary human macrophages (D) were treated with or without TNF-α (10 ng/ml) for 3 hours. Re-ChIP assays were performed with indicated antibodies. (E) THP-1 cells were treated with or without LPS (100 ng/ml) for 6 hours. Re-ChIP assays were performed with indicated antibodies. (E,F) THP-1 cells were transfected with indicated siRNA followed by treatment with TNF-α (10 ng/ml) for 6 hours. ChIP assays were performed with anti-MKL1 (E) or anti-p65 (F). (H) THP-1 cells were transfected with indicated siRNA followed by treatment with LPS (100 ng/ml) for 6 hours. ChIP assays were performed with anti-MKL1 or anti-p65.