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. 2017 Mar 23;7:378. doi: 10.1038/s41598-017-00329-y

Figure 4.

Figure 4

The function of pyrogallol (PG) and riboflavin (RF) on the catalytic activity of PLDz. (A) Effects of autoxidation of PG and photo-oxidation of RF on the cleavage reaction of PLDz. Reaction condition: 0.4 μM PLDz, 100 μM PG (RF), 100 μM Cu2+ (Fe2+), 300 mM NaCl and 50 mM Tris-HCl (pH 7.0) at 23 °C for 2 hr. The reaction system containing RF need to performed under a sunlight lamp. (B) Effects of SOD on the cleavage reaction of PLDz in the presence of H2O2. Reaction condition: 0.4 μM PLDz, 100 μM PG (RF), 0.3 U/μl SOD, 300 mM NaCl and 50 mM Tris-HCl (pH 7.0) at 23 °C for 2 hr. Cropped gels are used in Fig. 4A,B, their full-length gels are presented in Supplementary Figure 9.