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. 2017 Mar 23;7:378. doi: 10.1038/s41598-017-00329-y

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© The Author(s) 2017

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Effect of pyrogallol (PG) on a fluorescent labeled trans-PLDz. (A) Design of a fluorescent labeled trans-PLDz. C indicates Cy3, B indicates BHQ2. (B) Effects of CAT/SOD on the cleavage reaction of PLDz in the presence of PG. Reaction condition: 50 nM PL_B, 50 nM S_BC, 10 μM PG (50 mU/μl CAT, 5 mU/μl SOD), 300 mM NaCl and 50 mM MES (pH 6.0) at 37 °C for 1 hr.