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. 2017 Mar 21;7:240. doi: 10.1038/s41598-017-00393-4

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Autophagy is dispensability for steroidogenic cell growth. (A–D) Depletion of Beclin 1 does not affect steroidogenic cell growth. (A and C) Whole cell extracts of siRNA against Beclin1-treated TM3 (A) or Y1 (C) cell lines were analyzed by immunoblot with antibodies against Beclin1, α-tubulin, and γ-tubulin. NS: non-specific signal. (B and D) Quantitation of cell numbers of Beclin1 depleted TM3 (B) or Y1 (D) cell lines. (E,F) Depletion of Atg7 does not affect TM3 cell growth. (E) Whole cell extracts of lentivirus containing shRNA against Atg7 (shAtg7#1-4)-infected TM3 cell line were analyzed by immunoblot with antibodies against Atg7 and actin. (F) Quantitation of cell numbers of Atg7 depleted TM3 cell line. (G) Treatment of cells with autophagic inhibitor 3MA (2 mM) does not affect cell growth. Quantitation of cell numbers of 3MA-treated Y1 cells. These results are mean +/− SD from three independent experiments. n.s.: no significance.