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. 2017 Mar 15;7:183. doi: 10.1038/s41598-017-00317-2

Figure 4.

Figure 4

Methylation detection of the p16 DNA gene fragment. (a–d) Representative signal events and the histograms of target-probe DNA hybrids. A universal DNA probe (hp, blue colour) was used to identify and detect the methylation of different P16 gene fragments (black colour) containing zero 5′-methylcytosine (0 mC T) (a), one 5′-methylcytosine (1 mC T) (b), two 5′-methylcytosines (2 mC T) (c), and three 5′-methylcytosines (3 mC T) (d). Red represents the methylated points. (e,f) The peak values of log (dwell time) histogram (e) and mean interevent interval of hybrid DNA translocation events (f) for 0 mC T-hp, 1 mC T-hp, 2 mC T-hp and 3 mC T-hp. (g) f sig shows the concentration dependence of target DNA from 15 to 210 nM. Inset: the plot of f sig as a function of target DNA from 15 to 60 nM. Experimental conditions are the same as Fig. 1 except the applied voltage was +60 mV.