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. 2017 Apr 7;7:719. doi: 10.1038/s41598-017-00350-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dorsal spinal CSF-cNs also exhibit ultrastructural properties of sensory neurons. (a) Transverse section of the spinal cord showing restricted deposition of DAB in a dorsal CSF-cN. (b) Overall view of a DAB⁺ dorsal CSF-cN contacting the central canal (cc). (c,d) Dorsal CSF-cNs bear at the apical pole multiple microvilli (arrowheads). (d,e) In the apical pole is located a cilium (arrow) with two central microtubule singlets along the axoneme (double arrowhead), reminiscent of a motile cilium. (f) Dorsal CSF-cN also exhibit LGV distributed in the cytoplasm (dotted arrows). Scale bar = 10 μm (a), 2 μm (b), 1 µm (c,f) and 500 nm (d,e).