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. 2017 Apr 7;7:719. doi: 10.1038/s41598-017-00350-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ventral and dorsal CSF-cNs project onto distinct neuronal populations. (a–d) Z projection stacks showing contact from ventral and dorsal CSF-cNs onto different spinal targets. (a) Lateral view of a ventral CSF-cN (magenta, arrow) contacting 2 CaP motor neurons (identified based on their location within the segment) labelled in green in the Tg(parg ^mnet2 -GFP) transgenic line (a1,a2, double headed arrows). (b) Dorsal CSF-cN (green, arrowhead) contacting a putative V0-v interneuron (magenta, based on its dorso-ventral and lateral location) in the Tg(vglut2a:DsRed) transgenic line. (c,d) Ventral (c, arrow) and dorsal (d, arrowhead) CSF-cNs (magenta) contact CoPA sensory interneurons (green) labelled in the Tg(tbx16-GFP) transgenic line. Boxes with close-ups highlight contacts between the CSF-cN and its target. Scale bar = 20 µm (a–d) and 5 µm (a1–d1).