Figure 1.

Schematic drawing representing the herein presented methodology. Briefly, an established triple cell co-culture model of the human lung epithelial tissue barrier (consisting of an epithelial cell layer complimented with human blood monocyte derived macrophages and dendritic cells on the apical and basolaterial sides respectively), cultured on a micro-porous membrane insert is detached to form a cell suspension using a reproducible method based upon a short Trypsin-EDTA treatment. After successful detachment, the multi-cell suspension can then be analysed via multi-colour flow cytometry (FACS) to gain a perspective upon the status of each specific cell type of the co-culture system.